см. напр. использование аналогичных подходов для оптимизации антител:

Так как одновременная оптимизация всех остатков CDR невозможна из-за ограничений размера тестируемых библиотек, для решения этой проблемы используют три подхода: последовательную («CDR walking») модификацию остатков CDR [106], параллельную модификацию остатков CDR и рандомизацию некоторых CDR-петель.
https://www.fbras.ru/wp-content/uploads/2017/10/Altschuler.p...

In saturation mutagenesis, one or more positions along the protein sequence are identified as likely to accommodate beneficial mutations and are then randomized, i.e., the amino acids at these positions are replaced by random ones. The randomization originates at the DNA level, typically via degenerate primers containing a mixture of sequences at the chosen codons. To decrease the chances of introducing a premature stop codon, reduced codon sets are often used: NNB, NNS, and NNK codons (where N = A/C/G/T, B = C/G/T, S = C/G, and K = G/T) are popular choices that still encode all 20 amino acids, but the use of codon sets encoding fewer amino acids has been also advocated
https://aem.asm.org/content/78/1/258